Phosphoproteomics workflows generally require around 1 mg of sample lysate, use urea-based protein digestion, followed by peptide desalting and lyophilization, peptide fractionation using high-pH reversed-phase chromatography. Phosphopeptides are enriched by TiO2, sometimes multiple times, resulting in numerous LC-MS/MS measurements per biological sample to be analyzed.
We’re always trying to provide the highest quality proteomics data in the field by using state-of-the-art Mass Spectrometers to the most advanced platforms and constantly optimizing our sample preparation methods based on the latest development of technologies.
MS Equipment available:
Step 1 -> Please get in touch to discuss experimental setup and sample preparation
Step 2 -> We send you the sample preparation protocol and checklist
Step 3 -> You prepare and ship the samples to PEPSCOPE
Step 4 -> We process your samples
Step 5 -> We analyze the results and prepare a comprehensive report
Step 6 -> You get your results
Measuring Target Phosphorylation through Mass Spectrometry
PEPSCOPE’s comprehensive phosphoproteomics services to our clients include mass spectrometry-based proteomics solutions for analyses of cell lines and tissue. This service permits the identification and quantification of proteins, phosphopeptides (abundance change and phosphorylation status) and kinases in a single experiment.
Depending on the research question we:
Our solutions are focused on the customer’s needs and enable insights into the mode of action of post-translational phosphorylation modifications. Through our strong and long term collaboration with the Erasmus Medical Center in Rotterdam (group of Theo Luider) we have access to their thorough knowledge/experience and skills.
Our services include:
PEPSCOPE offers multiplexing up to 10 different samples through Tandem-Mass-Target (TMT) labeling. Through the Erasmus MC, Pepscope has many years experience using TMT labeling.