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Applications

Applications

Activity-based Kinome Profiling

Phosphoproteomic Profiling

Kinase target discovery

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Kinase inhibitor studies

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Purified kinase characterization

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Investigating combination therapies

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Activity-based Kinome Profiling

Kinome Profiling microarrays contain a broad set of substrate peptides. These substrates are derived from proteins involved in all relevant signal transduction pathways.

Kinome profiling of cell- and tissue lysates offers:

  1. An in-depth characterization of the activity of protein kinases pathways in cell- and tissue lysates.
  2. Insight in the substrate preference of specific kinases by interrogation of up to 1.024 different kinase substrates in a single experiment

You get insight in up- and downregulated pathways, providing valuable information about signaling pathways involved in the mode of action of kinase inhibitors, disease mechanisms or physiological processes.

 

Your questions and results

Our Kinome Profiling technology is highly suitable to answer questions such as:

  • Mode of action and off-target effects of kinase inhibitors
  • Comparing active kinases in healthy and diseased states
  • Investigating drug resistance

 

Sample types

PEPSCOPE’s profiling arrays are compatible with a multitude of cell and tissue samples, such as:

  • Fresh tumor tissue samples
  • Cultured cells
  • Biopsies
  • Kinase mixtures
  • Small organisms, such as zebrafish and drosophila embryos and nematodes

 

Read more about Kinome Profiling >

MS-based Phosphoproteomic Profiling

Our phosphoproteomics services include MS-based proteomics solutions for analyses of cells and tissues. This service allows for the identification and quantification of proteins, phosphopeptides and kinases in a single experiment. We have a long-standing collaboration with the group of dr. Theo Luider of the Erasmus Medical Center in Rotterdam (NL).

 

Our services include:

  1. Contract and custom research
  2. Consultancy
  3. Assay development and validation

 

PEPSCOPE offers multiplexing of up to 10 different samples through Tandem-Mass-Target (TMT) labeling.

 

Read more about Phosphoproteomic Profiling >